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Cell Culture Planner

Vessel and cell-line reference tables (45 lines), a split/passage planner with feasibility check, and growth projection with chart. The same math as the Excel workbook, live in your browser.

What this is for: Plans a passage (expected harvest, transfer volumes, implied split ratio, feasibility) and projects growth to confluence, over a 45-line cell-line reference table.

The formula: harvest = confluent density (cells/cm²) × area (cm²) × confluency %; N(t) = N0 × 2^(t ÷ doubling time)

How to use:

  1. In the Split / Passage Planner, pick the cell line and source vessel and enter the current confluency.
  2. Pick the destination vessel, number of destination vessels, target seeding percentage, and your counted stock concentration.
  3. Read the expected harvest, transfer volumes, implied split ratio, maximum vessels covered, and feasibility.
  4. In Growth Projection, pick the cell line, enter the seeding number N₀ and vessel, and set a custom time to read the projected count and hours to 90% confluence.
  5. Use the vessel and cell-line reference tables below to check the areas, densities, and doubling times in use.

Split / Passage Planner

Pick the cell line and source vessel: expected harvest = line density × vessel area × current confluency. Then pick the destination: cells needed, how much of your counted harvest suspension to transfer, the implied split ratio, and whether the harvest is enough.

%
%
Confluent density (cells/cm²)
Doubling time (h)
Typical split (1:X)
Source growth area (cm²)
EXPECTED HARVEST (cells)
Destination growth area (cm²)
Cells needed per destination vessel
Total cells needed
Suspension to transfer — total (mL)
Suspension to transfer — per vessel (mL)
Implied split ratio (1 : X)
Max destination vessels from this harvest
Feasibility

QUICK TABLE — one confluent source vessel seeds how many of each destination (at the target % above)?

Destination vesselCells per vesselVessels covered

Growth Projection — N(t) = N0 × 2^(t / doubling time)

Pick a cell line (doubling time fills in) and a seeding number; projected counts and the estimated time to 90% confluence in the chosen vessel.

h
Doubling time (h)
Confluent density (cells/cm²)
Vessel capacity at confluence (cells)
Estimated hours to 90% confluence
Projected cells at the custom time
Time (h)Projected cells

Reference: Culture Vessels

Generic confluent density used for the last column: 100,000 cells/cm². The Split planner uses each cell line's own density from the Cell lines table.

VesselGrowth area (cm²)Typical media (mL)Cells at 100% confluence (generic)

Reference: Cell Lines — TYPICAL published values, adjust for your lab

Densities and doubling times are widely published typical values; your subclone, medium and conditions will shift them — measure and overwrite in the workbook.

Cell lineCells/cm² at confluenceDoubling time (h)Typical splitNotes
Prefer to work offline or keep a copy? The download link is right below.
Download the Cell Culture Planner workbook (.xlsx)
Notes & limits (from the workbook): Vessels: “Cells at confluence” in the reference table uses a generic 100,000 cells/cm²; the Split planner uses each cell line’s own density from the Cell lines sheet. Cell-line densities and doubling times are widely published typical values — your subclone, medium and conditions will shift them, so measure and overwrite. Jurkat is a suspension line: its density is per mL, not per cm²; keep 5e5–2e6/mL.