Buffers & Solutions
Molarity & Mass Calculator
Pick a chemical, set molarity and volume — get the grams to weigh. Reverse mode, % w/v and % v/v helpers included.
Dilution Calculator (C1V1 = C2V2)
Solve for any one of C1, V1, C2, V2, plus a serial-dilution planner with the concentration at every step.
Buffer & Solution Maker
Up to 8 components with automatic molecular-weight lookup and one shared volume. PBS, TAE 50X and TBS presets included.
Chemicals Database
About 130 common lab chemicals with formula, molecular weight, CAS number and form notes — the same database that powers the dropdowns.
Antibiotic & Media Additives
Ampicillin, kanamycin, IPTG, X-gal and more: enter your culture volume and get the µL of stock to add.
Serial Dilution Scheme Designer
How many steps to reach your target concentration, and the transfer + diluent volume for every tube.
Molecular Biology & DNA/RNA
DNA & Primer Calculator
Copy number from ng and length, primer reconstitution (nmol → µL), and µg ↔ pmol for dsDNA.
DNA/RNA Concentration from A260
Concentration and total yield from an A260 reading, with A260/A280 and A260/A230 purity interpretation.
Oligo / Primer Tm Calculator
Paste a primer sequence: base counts, GC%, Wallace and salt-adjusted Tm, and a suggested annealing temperature.
PCR Mastermix Calculator
Per-reaction components scaled to your reaction count plus overage — water balances automatically.
Restriction Digest Calculator
Single and double digests: µL of DNA, enzyme, buffer and water for the amount of DNA you want to cut.
Restriction Digestion Calculator (Enzyme Database)
Full digest planning over a 94-enzyme database (HF/plain versions, buffer, temperature, methylation): unit math with a glycerol warning, double-digest compatibility, and cutting frequency.
Cloning
Ligation & Gibson/HiFi Calculator
Insert ng and µL at 1:1 to 10:1 molar ratios (3:1 highlighted), plus a pmol-based Gibson/HiFi assembly sheet.
Transformation Efficiency
CFU/µg from colonies counted, DNA used and the fraction of the recovery you plated.
Cell Culture & Transfection
Hemocytometer Cell Counting
Live/dead counts from the 4 large squares → cells/mL, total cells and viability %.
Cell Seeding Calculator
Cells per well, plate format and stock concentration → stock + media volumes with overage.
Cell Culture Planner
Vessel and cell-line reference tables (16 lines), a split/passage planner with feasibility check, and growth projection with chart.
Transfection Calculator (PEI & Lipofectamine)
Pick a vessel and the DNA, PEI (1:3 and 1:6) or Lipofectamine 3000/P3000 volumes fill in — including 4-plasmid co-transfection shares.
Virology
Plaque Assay PFU Calculator
Plaques, dilution and volume plated → PFU/mL per replicate, mean titer, SD, and a 10–100 countable-range flag.
MOI Calculator
Cells + titer + desired MOI → µL of virus stock to add; reverse mode gives the MOI you actually got.
TCID50 Calculator (Spearman–Kärber)
Wells positive/total per dilution → log10 TCID50/mL with the standard Kärber formula in cells.
ELISA & Assays
ELISA Analysis (4PL + Endpoint Titer)
Blank subtraction, duplicate CV flags, linear and 4PL standard curves with live chart, sample concentrations by 4PL inversion, and an endpoint-titer sheet with Reed–Muench-style interpolation.
Standard Curve & Linear Regression
XY regression with slope, intercept, R² and Pearson p, plus an ELISA/Bradford standard-curve sheet that converts absorbances to concentrations.
Enzyme Kinetics (Michaelis–Menten)
Initial rates at several substrate concentrations → Lineweaver–Burk fit for Km and Vmax with R², kcat from your enzyme concentration, a predicted rate at any [S], and two live charts.
Protein
Protein Concentration from A280
Beer–Lambert from the extinction coefficient and MW, or the E1% mode (e.g. IgG) — mg/mL, µM and total yield.
Statistics & Graphs — the mini-Prism series
Descriptive Statistics
Up to 6 groups × 20 values: n, mean, SD, SEM, median, range, 95% CI, and a bar chart of means ± SEM.
t-Tests (Student, Welch, Paired)
Three sheets with t, df, p-value, Cohen's d, significance stars and a means chart with error bars.
One-Way ANOVA
Full ANOVA table from cell formulas, plus a pairwise post-hoc p-value matrix with Bonferroni guidance and a chart.
Dose-Response & IC50 (4PL)
Log-dose vs response scatter with an adjustable 4-parameter logistic curve, plus an interpolation-based IC50.
qPCR ΔΔCt Analysis
Ct target/reference per sample → ΔCt, ΔΔCt and 2^-ΔΔCt fold changes with a group chart.
Kaplan–Meier Survival
Two-group survival from per-animal time/status data: KM step curves and median survival per group.
Chi-Square Contingency Tables
2×2 (with odds ratio and relative risk + 95% CI) and up to 4×4 tables, expected counts computed in cells.
Growth Curves
Time-course means ± SD with a live line chart, AUC per condition, and doubling time from two time points.
Sample Size & Power
How many per group for comparing two means or two proportions, with dropout inflation — plus the smallest difference a fixed n can detect.
Flow Cytometry
Flow Cytometry Calculator
Four sheets: staining-cocktail builder (panel µL per test × samples + overage, cells per test), absolute counts from counting beads, event rate & acquisition time with dilution to target, and antibody titration by stain index.
Pharmacology & Dosing
Pharmacokinetics (AUC, Clearance, Half-Life)
Trapezoid AUC from a concentration–time table, terminal half-life, clearance and volume, infusion steady state and loading dose — plus Cockcroft–Gault and CKD-EPI 2021 kidney function.
BSA & Allometric Dosing
Body surface area (Mosteller + Du Bois), mg/m² dosing to mL from stock, the FDA Km table for species dose conversion, and power-law allometric scaling.
Ecology & Evolution
Biodiversity Indices
Species counts → Shannon, Simpson, Pielou evenness, richness estimators and Chao1, with a counts chart; Bray–Curtis, Sørensen and Jaccard similarity between two sites on the second sheet.
Ecology & Population Calculator
Mark–recapture population estimates (Lincoln–Petersen and Chapman, with 95% CI), quadrat density and frequency, and exponential vs logistic growth with a live chart.
Hardy–Weinberg Calculator
Genotype counts → allele frequencies, expected counts and a chi-square equilibrium test; plus carrier frequency from the recessive phenotype frequency.
Epidemiology & Diagnostics
Epidemiology & Diagnostic Tests
Sensitivity, specificity, predictive values and likelihood ratios from a 2×2 table (re-scored at any prevalence), relative risk with 95% CI, odds ratio, NNT, and incidence / prevalence / attack rates.
General Lab & Microbiology
Centrifuge RCF ⇄ RPM Converter
Rotor radius + RPM → g-force, and the RPM needed for a target RCF.
Bacterial CFU/mL Calculator
Colonies, dilution and volume plated → CFU/mL with the 30–300 countable-range flag.
Bacterial Growth: McFarland, OD600, Doubling
McFarland-to-CFU/mL reference, OD600 → cells/mL with an editable factor, and doubling time from two OD readings.
Animal Dose Calculator
mg/kg dose + stock concentration + each animal's weight → mg and injection µL per animal, with prep totals.
About
Built by a working virologist who got tired of re-deriving the same bench math and of statistics software priced for institutions, not people. Every formula is visible in the cell — check it, change it, teach with it. If a file saves you time, the coffee button above is the only paywall there will ever be, and it's optional.
Request a Calculator or Report an Error
Missing a calculation you run every week? Found a formula that looks wrong? Tell us here. Requests are read by a human — good ones get built and verified the same way as the rest of the library, and fixes ship as revisions to the workbook.
Community Updates
- Requests are open — the first community-built calculators will appear here.